[PDF][PDF] Silver staining as a tool for neurotoxic assessment

CA Beltramino, JS de Olmos, F Gallyas… - NIDA Res …, 1993 - researchgate.net
CA Beltramino, JS de Olmos, F Gallyas, L Heimer, L Záborszky
NIDA Res Monogr, 1993researchgate.net
Neurotoxicology and neurotoxicity have become matters of great public concern, and efforts
have intensified to find reliable and convenient methods to identify those chemicals and
substances that are potentially harmful to health because of their toxic effect on the central
nervous system (CNS). A reliable answer is needed for the following question: Have the
basic units of the CNS (ie, the neurons) suffered irreversible damage as a result of the toxic
effect? In other words, the task is to find a handy method to detect signs of degeneration in …
Neurotoxicology and neurotoxicity have become matters of great public concern, and efforts have intensified to find reliable and convenient methods to identify those chemicals and substances that are potentially harmful to health because of their toxic effect on the central nervous system (CNS). A reliable answer is needed for the following question: Have the basic units of the CNS (ie, the neurons) suffered irreversible damage as a result of the toxic effect? In other words, the task is to find a handy method to detect signs of degeneration in neurons and to determine the location and extent of such neuronal degeneration in the CNS. The reduced silver methods’ have in the past been the most effective procedures for mapping experimentally induced neuronal degenerations, and the results demonstrated by modern silver methods indicate that they also have the qualities necessary to make them a powerful method for screening neuronal damage caused by various neurotoxic substances including drugs of abuse.
Since the early part of of this century, silver methods have been used with great success to stain both normal and pathologic components of nervous tissue (von Fajersztajn 1901; Bielschowsky 1904; Ram ny Cajal 1904). The ease with which various tissue components attract silver and the high-contrast microscopic images provided by metallic silver deposits are two of the most attractive features of silver impregnation.
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