HSP104 required for induced thermotolerance

Y Sanchez, SL Lindquist - Science, 1990 - science.org
Y Sanchez, SL Lindquist
Science, 1990science.org
A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a
deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-
type cells and died at the same rate when exposed directly to high temperatures. However,
when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as
did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells.
The results demonstrate that a particular heat shock protein plays a critical role in cell …
A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.
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