[HTML][HTML] Nitric oxide acts directly in the presynaptic neuron to produce long-term potentiationin cultured hippocampal neurons
O Arancio, M Kiebler, CJ Lee, V Lev-Ram, RY Tsien… - Cell, 1996 - cell.com
O Arancio, M Kiebler, CJ Lee, V Lev-Ram, RY Tsien, ER Kandel, RD Hawkins
Cell, 1996•cell.comNitric oxide (NO) has been proposed to act as a retrograde messenger during long-term
potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the
presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of
the synapse are accessible in cultured hippocampal neurons, we have used this preparation
to investigate the role of NO. We examined LTP following intra-or extracellular application of
an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that …
potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the
presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of
the synapse are accessible in cultured hippocampal neurons, we have used this preparation
to investigate the role of NO. We examined LTP following intra-or extracellular application of
an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that …
Abstract
Nitric oxide (NO) has been proposed to act as a retrograde messenger during long-term potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of the synapse are accessible in cultured hippocampal neurons, we have used this preparation to investigate the role of NO. We examined LTP following intra- or extracellular application of an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts as a retrograde messenger during LTP.
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