Characterisation of isolated α-synuclein filaments from substantia nigra of Parkinson's disease brain

RA Crowther, SE Daniel, M Goedert - Neuroscience letters, 2000 - Elsevier
RA Crowther, SE Daniel, M Goedert
Neuroscience letters, 2000Elsevier
The defining neuropathological deposits of Parkinson's disease, dementia with Lewy bodies
and multiple system atrophy are strongly immunoreactive for α-synuclein. We have shown
previously that isolated filaments from dementia with Lewy bodies and multiple system
atrophy brains are labelled in a characteristic fashion by a number of α-synuclein antibodies.
Here we have extracted filaments from substantia nigra of patients with idiopathic
Parkinson's disease. Antibodies directed against the carboxy-terminal region of α-synuclein …
The defining neuropathological deposits of Parkinson's disease, dementia with Lewy bodies and multiple system atrophy are strongly immunoreactive for α-synuclein. We have shown previously that isolated filaments from dementia with Lewy bodies and multiple system atrophy brains are labelled in a characteristic fashion by a number of α-synuclein antibodies. Here we have extracted filaments from substantia nigra of patients with idiopathic Parkinson's disease. Antibodies directed against the carboxy-terminal region of α-synuclein labelled isolated filaments along their entire lengths. By contrast, an antibody directed against the amino-terminal region of α-synuclein only labelled one filament end. These characteristics were identical to those of filaments extracted from brains of patients with dementia with Lewy bodies and multiple system atrophy.
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