A CFSE based assay for measuring CD4+ CD25+ regulatory T cell mediated suppression of auto-antigen specific and polyclonal T cell responses
K Venken, M Thewissen, N Hellings, V Somers… - Journal of …, 2007 - Elsevier
CD4+ CD25+ regulatory T cells (Tregs) are considered to play a key role as suppressors of
immune mediated reactions. The analysis of Treg function in patients with autoimmune,
allergic or oncogenic diseases has emerged over the past years. In the present study we
describe a CFSE based protocol to measure Treg mediated suppression of CD4+ T cells.
Measuring Treg suppressive capacity towards proliferation of anti-CD3 Ab stimulated CD4+
CD25− T cells in coculture experiments by means of a CFSE based and a classical [3H] …
immune mediated reactions. The analysis of Treg function in patients with autoimmune,
allergic or oncogenic diseases has emerged over the past years. In the present study we
describe a CFSE based protocol to measure Treg mediated suppression of CD4+ T cells.
Measuring Treg suppressive capacity towards proliferation of anti-CD3 Ab stimulated CD4+
CD25− T cells in coculture experiments by means of a CFSE based and a classical [3H] …