[HTML][HTML] Analysis of the Munc18b-syntaxin binding interface: use of a mutant Munc18b to dissect the functions of syntaxins 2 and 3
M Kauppi, G Wohlfahrt, VM Olkkonen - Journal of Biological Chemistry, 2002 - ASBMB
Munc18b is a mammalian Sec1-related protein that is abundant in epithelial cells and
regulates vesicle transport to the apical plasma membrane. We constructed a homology
model of Munc18b in complex with syntaxin 3 based on the crystal structure of the neuronal
Sec1· syntaxin 1A complex. In this model we identified all residues in the interface between
the two proteins that contribute directly to the interaction and mutagenized residues in
Munc18b to alter its binding to syntaxins 1A, 2, and 3. The syntaxin-binding properties of the …
regulates vesicle transport to the apical plasma membrane. We constructed a homology
model of Munc18b in complex with syntaxin 3 based on the crystal structure of the neuronal
Sec1· syntaxin 1A complex. In this model we identified all residues in the interface between
the two proteins that contribute directly to the interaction and mutagenized residues in
Munc18b to alter its binding to syntaxins 1A, 2, and 3. The syntaxin-binding properties of the …