Structure and function of phosphatidylserine-specific phospholipase A1

J Aoki, Y Nagai, H Hosono, K Inoue, H Arai - Biochimica et Biophysica Acta …, 2002 - Elsevier
J Aoki, Y Nagai, H Hosono, K Inoue, H Arai
Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2002Elsevier
Phospholipase A1 (PLA1) is an enzyme that hydrolyzes the sn-1 fatty acids from
phospholipids and produces 2-acyl-lysophospholipids. Although PLA1 activities are
detected in many tissues and cell lines, a limited number of PLA1s have been purified and
cloned so far. These include phosphatidylserine (PS)-specific PLA1 (PS-PLA1) from rat
platelets, PLA1 from vespid venom, and phosphatidic acid (PA)-preferential PLA1 (PA-
PLA1). Structurally, the former two PLA1s belong to the lipase family, where they form a …
Phospholipase A1 (PLA1) is an enzyme that hydrolyzes the sn-1 fatty acids from phospholipids and produces 2-acyl-lysophospholipids. Although PLA1 activities are detected in many tissues and cell lines, a limited number of PLA1s have been purified and cloned so far. These include phosphatidylserine (PS)-specific PLA1 (PS-PLA1) from rat platelets, PLA1 from vespid venom, and phosphatidic acid (PA)-preferential PLA1 (PA-PLA1). Structurally, the former two PLA1s belong to the lipase family, where they form a subfamily among the lipase family. An alignment of the PLA1s with other members of the lipase family revealed two molecular characteristics of PLA1: the presence of extremely short lids and deleted β9 loops. The two surface loops have been implicated in the ligand recognition in human pancreatic lipase (PL) and guinea pig PL-related protein 2. Under physiological conditions, accessibility of PS-PLA1 to its substrate is limited as it is a secreted enzyme and PS is normally located in the inner leaflet of the lipid bilayer. However, PS-PLA1 efficiently hydrolyzes PS exposed on the surface of cells such as apoptotic cells and activated platelets, and produces 2-acyl-lysophosphatidylserine (lysoPS), which is a lipid mediator for mast cells, T cells and neural cells. Identification of PS-PLA1 reveals the presence of PLA1 subfamily within the lipase family and suggests that PLA1 has a role in the production of lysophospholipid mediators.
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