Direct isolation of human central nervous system stem cells

N Uchida, DW Buck, D He… - Proceedings of the …, 2000 - National Acad Sciences
N Uchida, DW Buck, D He, MJ Reitsma, M Masek, TV Phan, AS Tsukamoto, FH Gage
Proceedings of the national academy of sciences, 2000National Acad Sciences
Stem cells, which are clonogenic cells with self-renewal and multilineage differentiation
properties, have the potential to replace or repair damaged tissue. We have directly isolated
clonogenic human central nervous system stem cells (hCNS-SC) from fresh human fetal
brain tissue, using antibodies to cell surface markers and fluorescence-activated cell sorting.
These hCNS-SC are phenotypically 5F3 (CD133)+, 5E12+, CD34−, CD45−, and CD24−/lo.
Single CD133+ CD34− CD45− sorted cells initiated neurosphere cultures, and the progeny …
Stem cells, which are clonogenic cells with self-renewal and multilineage differentiation properties, have the potential to replace or repair damaged tissue. We have directly isolated clonogenic human central nervous system stem cells (hCNS-SC) from fresh human fetal brain tissue, using antibodies to cell surface markers and fluorescence-activated cell sorting. These hCNS-SC are phenotypically 5F3 (CD133)+, 5E12+, CD34, CD45, and CD24−/lo. Single CD133+ CD34 CD45 sorted cells initiated neurosphere cultures, and the progeny of clonogenic cells could differentiate into both neurons and glial cells. Single cells from neurosphere cultures initiated from CD133+ CD34 CD45 cells were again replated as single cells and were able to reestablish neurosphere cultures, demonstrating the self-renewal potential of this highly enriched population. Upon transplantation into brains of immunodeficient neonatal mice, the sorted/expanded hCNS-SC showed potent engraftment, proliferation, migration, and neural differentiation.
National Acad Sciences