The VITAL assay: a versatile fluorometric technique for assessing CTL-and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo
IF Hermans, JD Silk, J Yang, MJ Palmowski… - Journal of …, 2004 - Elsevier
Assessment of cell-mediated toxicity has traditionally been achieved by measuring the
specific activity of enriched effector cell populations against antigen-loaded target cells
labeled with radioactive isotopes in vitro. Fluorometric techniques are viewed as a promising
alternative to the use of radioactive isotopes for these analyses. Direct assessment of
cytotoxicity in vivo can be achieved by monitoring survival of injected fluorescent targets
relative to a differentially labeled internal control population without specific antigen. We …
specific activity of enriched effector cell populations against antigen-loaded target cells
labeled with radioactive isotopes in vitro. Fluorometric techniques are viewed as a promising
alternative to the use of radioactive isotopes for these analyses. Direct assessment of
cytotoxicity in vivo can be achieved by monitoring survival of injected fluorescent targets
relative to a differentially labeled internal control population without specific antigen. We …