[HTML][HTML] Isolation and characterization of rat liver amphisomes: evidence for fusion of autophagosomes with both early and late endosomes
TO Berg, M Fengsrud, PE Strømhaug, T Berg… - Journal of Biological …, 1998 - ASBMB
Amphisomes, the autophagic vacuoles (AVs) formed upon fusion between autophagosomes
and endosomes, have so far only been characterized in indirect, functional terms. To enable
a physical distinction between autophagosomes and amphisomes, the latter were
selectively density-shifted in sucrose gradients following fusion with AOM-gold-loaded
endosomes (endosomes made dense by asialoorosomucoid-conjugated gold particles,
endocytosed by isolated rat hepatocytes prior to subcellular fractionation). Whereas …
and endosomes, have so far only been characterized in indirect, functional terms. To enable
a physical distinction between autophagosomes and amphisomes, the latter were
selectively density-shifted in sucrose gradients following fusion with AOM-gold-loaded
endosomes (endosomes made dense by asialoorosomucoid-conjugated gold particles,
endocytosed by isolated rat hepatocytes prior to subcellular fractionation). Whereas …