A rapid and efficient method for the generation and screening of monoclonal antibodies specific for cell surface antigens

A Mesci, JR Carlyle - Journal of immunological methods, 2007 - Elsevier
The generation of monoclonal antibodies (mAb) of desired specificity to cell surface antigens
can serve as a valuable tool to study protein expression and function. However, traditional
approaches to mAb generation usually involve large-scale protein purification and intensive
screening, and may not result in mAb specificities to the native protein of interest. We
describe a simple, inexpensive, high-throughput method for the generation and screening of
hybridomas secreting mAb specific for cell surface receptors. Intact reporter cells expressing …