[HTML][HTML] Raf-1/MEK/MAPK pathway is necessary for the G2/M transition induced by nocodazole
The dynamic balance between polymerization and depolymerization of microtubules is
critical for cells to enter and exit mitosis, and drugs that disrupt this balance, such as taxol,
colchicine, and nocodazole, arrest the cell cycle in mitosis. Although the Raf/MEK/MAPK
pathway can be activated by these drugs, its role in mitosis has not been addressed. Here,
we characterize activation of Raf/MEK/MAPK by nocodazole when mitosis is induced. We
find that at early time points (up to 3 h) in nocodazole induction, Raf/MEK/MAPK is activated …
critical for cells to enter and exit mitosis, and drugs that disrupt this balance, such as taxol,
colchicine, and nocodazole, arrest the cell cycle in mitosis. Although the Raf/MEK/MAPK
pathway can be activated by these drugs, its role in mitosis has not been addressed. Here,
we characterize activation of Raf/MEK/MAPK by nocodazole when mitosis is induced. We
find that at early time points (up to 3 h) in nocodazole induction, Raf/MEK/MAPK is activated …