Heritable transgenesis of Parastrongyloides trichosuri: a nematode parasite of mammals
WN Grant, SJM Skinner, J Newton-Howes… - International journal for …, 2006 - Elsevier
Germline transformation of a parasitic nematode of mammals has proven to be an elusive
goal. We report here the heritable germline transformation of Parastrongyloides trichosuri, a
nematode parasite whose natural hosts are Australian possums of the genus Trichosurus.
This parasite can undergo multiple free-living life cycles and these replicative cycles can be
maintained indefinitely in the laboratory. Transformation was achieved by microinjection of
DNA into the ovary syncytium of either free-living or parasitic adult females. By selecting for …
goal. We report here the heritable germline transformation of Parastrongyloides trichosuri, a
nematode parasite whose natural hosts are Australian possums of the genus Trichosurus.
This parasite can undergo multiple free-living life cycles and these replicative cycles can be
maintained indefinitely in the laboratory. Transformation was achieved by microinjection of
DNA into the ovary syncytium of either free-living or parasitic adult females. By selecting for …