T cell-mediated tumor rejection displays diverse dependence upon perforin and IFN-γ mechanisms that cannot be predicted from in vitro T cell characteristics

L Peng, JC Krauss, GE Plautz, S Mukai… - The Journal of …, 2000 - journals.aai.org
L Peng, JC Krauss, GE Plautz, S Mukai, S Shu, PA Cohen
The Journal of Immunology, 2000journals.aai.org
Experimental pulmonary metastases have been successfully treated by adoptive transfer of
tumor-sensitized T cells from perforin knockout (KO) or Fas/APO-1 ligand KO mice,
suggesting a prominent role for secretion of cytokines such as IFN-γ. In the present study we
confirmed that rejection of established methylcholanthrene-205 (MCA-205) pulmonary
metastases displayed a requirement for T cell IFN-γ expression. However, this requirement
could be obviated by transferring larger numbers of tumor-sensitized IFN-γ KO T cells or by …
Abstract
Experimental pulmonary metastases have been successfully treated by adoptive transfer of tumor-sensitized T cells from perforin knockout (KO) or Fas/APO-1 ligand KO mice, suggesting a prominent role for secretion of cytokines such as IFN-γ. In the present study we confirmed that rejection of established methylcholanthrene-205 (MCA-205) pulmonary metastases displayed a requirement for T cell IFN-γ expression. However, this requirement could be obviated by transferring larger numbers of tumor-sensitized IFN-γ KO T cells or by immunosensitizing sublethal irradiation (500 rad) of the host before adoptive therapy. Extrapulmonary tumors (MCA-205 sc and intracranial) that required adjunct sublethal irradiation for treatment efficacy also displayed no requirement for host or T cell expression of IFN-γ. Nonetheless, rejection of MCA-205 sc tumors and ip EL-4 tumors, but not MCA-205 pulmonary or intracranial tumors, displayed a significant requirement for T cell perforin expression (ie, CTL participation). The capacity of T cells to lyse tumor targets and secrete IFN-γ in vitro before adoptive transfer was nonpredictive of the roles of these activities in subsequent tumor rejection. Adoptive therapy studies employing KO mice are therefore indispensable for revealing a diversity of tumor rejection mechanisms that may lack in vitro correlation due to delays in their induction. Seemingly contradictory KO data from different studies are reconciled by the capacity of anti-tumor T cells to rely on alternative mechanisms when treated in larger numbers, the variable participation of CTL at different anatomic locations of tumor, and the apparent capacity of sublethal irradiation to provide a therapeutic alternative to host or T cell IFN-γ production.
journals.aai.org