Characterization of a class of cationic proteins that specifically interact with intermediate filaments.

PM Steinert, JS Cantieri, DC Teller… - Proceedings of the …, 1981 - National Acad Sciences
PM Steinert, JS Cantieri, DC Teller, JD Lonsdale-Eccles, BA Dale
Proceedings of the National Academy of Sciences, 1981National Acad Sciences
We describe a class of cationic structural proteins that associate specifically with
intermediate filaments (IF) but not with other types of cytoskeletal proteins. These proteins,
for which the term filaggrin is introduced, are isolated from the stratum corneum of
mammalian epidermis. They are species-distinct proteins; for example, rat and mouse
filaggrin have different molecular weights and amino acid compositions, but are
nevertheless chemically and functionally ver similar. They interact in vitro with the IF several …
We describe a class of cationic structural proteins that associate specifically with intermediate filaments (IF) but not with other types of cytoskeletal proteins. These proteins, for which the term filaggrin is introduced, are isolated from the stratum corneum of mammalian epidermis. They are species-distinct proteins; for example, rat and mouse filaggrin have different molecular weights and amino acid compositions, but are nevertheless chemically and functionally ver similar. They interact in vitro with the IF several different types of cells to form large fibers or macrofibrils in which many IF are highly aligned in parallel arrays. Stoichiometric analyses suggest that two molecules of filaggrin bind to each three-chain building block of the IF, possibly by ionic interactions with the coiled-coil alpha-helical regions of the IF.
National Acad Sciences