[HTML][HTML] Huntingtin interacting protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain
V Legendre-Guillemin, M Metzler, JF Lemaire… - Journal of Biological …, 2005 - ASBMB
Huntingtin interacting protein 1 (HIP1) is a component of clathrin coats. We previously
demonstrated that HIP1 promotes clathrin assembly through its central helical domain,
which binds directly to clathrin light chains (CLCs). To better understand the relationship
between CLC binding and clathrin assembly we sought to dissect this interaction. Using C-
terminal deletion constructs of the HIP1 helical domain, we identified a region between
residues 450 and 456 that is required for CLC binding. Within this region, point mutations …
demonstrated that HIP1 promotes clathrin assembly through its central helical domain,
which binds directly to clathrin light chains (CLCs). To better understand the relationship
between CLC binding and clathrin assembly we sought to dissect this interaction. Using C-
terminal deletion constructs of the HIP1 helical domain, we identified a region between
residues 450 and 456 that is required for CLC binding. Within this region, point mutations …