Large scale purification and immunolocalization of bovine uroplakins I, II, and III. Molecular markers of urothelial differentiation.
The differentiation of mammalian urothelium culminates in the formation of asymmetrical unit
membrane (AUM). Using gradient centrifugation and detergent wash, we purified milligram
quantities of AUMs which, interestingly, contained three major proteins (15, 27, and 47 kDa)
that appeared to be identical to the three immunoaffinity purified, putatively AUM-associated
proteins that we described earlier (Yu, J., Manabe, M., Wu, X.-R., Xu, C., Surya, B., and Sun,
T.-T.(1990) J. Cell Biol., 111, 1207-1216). Peptide mapping and immunoblotting established …
membrane (AUM). Using gradient centrifugation and detergent wash, we purified milligram
quantities of AUMs which, interestingly, contained three major proteins (15, 27, and 47 kDa)
that appeared to be identical to the three immunoaffinity purified, putatively AUM-associated
proteins that we described earlier (Yu, J., Manabe, M., Wu, X.-R., Xu, C., Surya, B., and Sun,
T.-T.(1990) J. Cell Biol., 111, 1207-1216). Peptide mapping and immunoblotting established …