[18] Quantification of β-sheet amyloid fibril structures with thioflavin T

H LeVine III - Methods in enzymology, 1999 - Elsevier
Publisher Summary Despite the presence of a significant amount of carbohydrate in these
fibrils, the staining reaction was eventually shown to be because of the protein component.
The histologic benzothiazole dyes thioflavin S (ThS) and thioflavin T (ThT) under the
appropriate conditions selectively stain amyloid structures in a number of pathological
settings, as does the diazobenzidine sulfonate dye, Congo red, which is also birefringent
when bound to fibrils. Phorwhite BBU, Sirius Red, and several other fluorescent and …