An Fgf8 mutant allelic series generated by Cre-and Flp-mediated recombination

EN Meyers, M Lewandoski, GR Martin - Nature genetics, 1998 - nature.com
Nature genetics, 1998nature.com
We describe a strategy for generating an allelic series of mutations at a given locus that
requires the production of only one targetted mouse line. The 'allelogenic'mouse line we
produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by
mating to ere transgenic animals. The hypomorphic allele can also be reverted to wild-type
by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line
suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying …
Abstract
We describe a strategy for generating an allelic series of mutations at a given locus that requires the production of only one targetted mouse line. The ‘allelogenic’ mouse line we produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by mating to ere transgenic animals. The hypomorphic allele can also be reverted to wild-type by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying different combinations of these alleles revealed requirements for Fgf8 gene function during gastrulation, as well as cardiac, craniofacial, forebrain, midbrain and cerebellar development.
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