Molecular cloning of glucokinase cDNA. Developmental and dietary regulation of glucokinase mRNA in rat liver.
PB Iynedjian, C Ucla, B Mach - Journal of Biological Chemistry, 1987 - ASBMB
A rat liver cDNA library enriched for glucokinase sequences was constructed using the
phage expression vector lambda gt11 and screened with an antiserum to glucokinase. A
positive phage clone termed lambda-GK223 was isolated by several rounds of plaque
purification. When introduced in the high frequency lysogenization strain Y1089, the phage
was shown to encode a fusion protein containing epitopes specific to rat liver glucokinase.
The 1800-base pair cDNA insert of lambda-GK223 was subcloned in a pUC plasmid, and a …
phage expression vector lambda gt11 and screened with an antiserum to glucokinase. A
positive phage clone termed lambda-GK223 was isolated by several rounds of plaque
purification. When introduced in the high frequency lysogenization strain Y1089, the phage
was shown to encode a fusion protein containing epitopes specific to rat liver glucokinase.
The 1800-base pair cDNA insert of lambda-GK223 was subcloned in a pUC plasmid, and a …