[HTML][HTML] Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries
Cytochrome b of human neutrophils is the central component of the microbicidal NADPH-
oxidase system. However, the folding topology of this integral membrane protein remains
undetermined. Two random-sequence bacteriophage peptide libraries were used to map
structural features of cytochrome b by determining the epitopes of monoclonal antibodies
(mAbs) 44.1 and 54.1, specific for the p22 phox and gp91 phox cytochrome b chains,
respectively. The unique peptides of phage selected by mAb affinity purification were …
oxidase system. However, the folding topology of this integral membrane protein remains
undetermined. Two random-sequence bacteriophage peptide libraries were used to map
structural features of cytochrome b by determining the epitopes of monoclonal antibodies
(mAbs) 44.1 and 54.1, specific for the p22 phox and gp91 phox cytochrome b chains,
respectively. The unique peptides of phage selected by mAb affinity purification were …