In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and
are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in
a preformed isosmotic continuous gradient. Because of the large difference in density
between peroxisomes and the next densest organelle (mitochondria), a density barrier is
effective. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in
Percoll®, there is no contamination from endoplasmic reticulum.