Complexes of plasmid DNA with basic domain 47-57 of the HIV-1 Tat protein are transferred to mammalian cells by endocytosis-mediated pathways
IA Ignatovich, EB Dizhe, AV Pavlotskaya… - Journal of Biological …, 2003 - ASBMB
Arginine-rich peptides, penetratins, as part of a number of cellular and viral proteins, can
penetrate across plasma membrane directly, without participation of endocytosis. We show
that one of penetratins, the basic domain 47-57 of human immunodeficiency virus, type 1,
transcription factor Tat (Tat peptide), is able to interact with plasmid DNA electrostatically.
These interactions result in formation of polyelectrolytic complexes at various
negative/positive charge ratios of plasmid DNA and Tat peptide. Plasmid DNA is capable of …
penetrate across plasma membrane directly, without participation of endocytosis. We show
that one of penetratins, the basic domain 47-57 of human immunodeficiency virus, type 1,
transcription factor Tat (Tat peptide), is able to interact with plasmid DNA electrostatically.
These interactions result in formation of polyelectrolytic complexes at various
negative/positive charge ratios of plasmid DNA and Tat peptide. Plasmid DNA is capable of …
