[HTML][HTML] Endothelial nitric oxide synthase is regulated by tyrosine phosphorylation and interacts with caveolin-1
G García-Cardeña, R Fan, DF Stern, J Liu… - Journal of Biological …, 1996 - ASBMB
The regulation of endothelial nitric oxide synthase (eNOS) by phosphorylation is poorly
understood. Here, we demonstrate that eNOS is tyrosine-phosphorylated in bovine aortic
endothelial cells (BAEC) using 32 P metabolic labeling followed by phosphoamino acid
analysis and by phosphotyrosine specific Western blotting. Treatment of BAEC with
hydrogen peroxide and the protein tyrosine phosphatase inhibitor, sodium orthovanadate,
increases eNOS tyrosine phosphorylation. Utilizing a novel immunoNOS assay, the increase …
understood. Here, we demonstrate that eNOS is tyrosine-phosphorylated in bovine aortic
endothelial cells (BAEC) using 32 P metabolic labeling followed by phosphoamino acid
analysis and by phosphotyrosine specific Western blotting. Treatment of BAEC with
hydrogen peroxide and the protein tyrosine phosphatase inhibitor, sodium orthovanadate,
increases eNOS tyrosine phosphorylation. Utilizing a novel immunoNOS assay, the increase …