Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors
HE Young, TA Steele, RA Bray… - … Record: An Official …, 2001 - Wiley Online Library
HE Young, TA Steele, RA Bray, J Hudson, JA Floyd, K Hawkins, K Thomas, T Austin…
The Anatomical Record: An Official Publication of the American …, 2001•Wiley Online LibraryThis study details the profile of 13 cell surface cluster differentiation markers on human
reserve stem cells derived from connective tissues. Stem cells were isolated from the
connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric
humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity
of the stem cells from each population. All populations contained lineage‐committed
myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as …
reserve stem cells derived from connective tissues. Stem cells were isolated from the
connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric
humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity
of the stem cells from each population. All populations contained lineage‐committed
myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as …
Abstract
This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage‐committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage‐uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin‐A, and HLA DR‐II. Anat Rec 264:51–62, 2001. © 2001 Wiley‐Liss, Inc.
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