The development of fibroblast colonies in monolayer cultures of guinea‐pig bone marrow and spleen cells

AJ Friedenstein, RK Chailakhjan… - Cell …, 1970 - Wiley Online Library
AJ Friedenstein, RK Chailakhjan, KS Lalykina
Cell Proliferation, 1970Wiley Online Library
In monolayer cultures of guinea‐pig bone marrow and spleen the development of discrete
fibroblast colonies takes place on days 9–12. The linear increase in the number of colonies
with increasing numbers of explanted cells and the distribution of male and female cells in
mixed cultures support the view that fibroblast colonies are clones. The concentration of
colony‐forming cells in bone marrow and spleen is approximately 10‐5. Bone marrow
culture (but not spleen culture) fibroblasts are capable of spontaneous bone formation in …
Abstract
In monolayer cultures of guinea‐pig bone marrow and spleen the development of discrete fibroblast colonies takes place on days 9–12. The linear increase in the number of colonies with increasing numbers of explanted cells and the distribution of male and female cells in mixed cultures support the view that fibroblast colonies are clones. The concentration of colony‐forming cells in bone marrow and spleen is approximately 10‐5. Bone marrow culture (but not spleen culture) fibroblasts are capable of spontaneous bone formation in diffusion chambers. Fibroblasts from both bone marrow and spleen cultures are inducible to osteogenesis in diffusion chambers in the presence of transitional epithelium.
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