[HTML][HTML] Cloning of the amino-terminal and 5′-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts
D Li, M Gallup, N Fan, DE Szymkowski… - Journal of Biological …, 1998 - ASBMB
To obtain gene regulatory sequence for the mucin gene MUC5AC, we have isolated the
MUC5AC amino terminus cDNA and 5′-flanking region. This was possible through the use
of rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) in which the
5′ sequence of the human gastric mucin cDNA HGM-1 (1) was used to design the first
MUC5AC-specific primer. Primers for subsequent rounds of RACE were designed from the
5′-ends of amplified RACE products. After five rounds of RACE-PCR, we could no longer …
MUC5AC amino terminus cDNA and 5′-flanking region. This was possible through the use
of rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) in which the
5′ sequence of the human gastric mucin cDNA HGM-1 (1) was used to design the first
MUC5AC-specific primer. Primers for subsequent rounds of RACE were designed from the
5′-ends of amplified RACE products. After five rounds of RACE-PCR, we could no longer …