Proteins mediate their biological function through interactions with other proteins. Therefore,
the systematic identification and characterization of protein-protein interactions have
become a powerful proteomic strategy to understand protein function and comprehensive
cellular regulatory networks. For the screening of valosin-containing protein, carboxyl
terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners, we
utilized a membrane-based array technology that allows the identification of human protein …