[HTML][HTML] Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q
B Marbois, P Gin, KF Faull, WW Poon, PT Lee… - Journal of Biological …, 2005 - ASBMB
Coenzyme Q (Q) is a redox active lipid essential for aerobic respiration in eukaryotes. In
Saccharomyces cerevisiae at least eight mitochondrial polypeptides, designated Coq1–
Coq8, are required for Q biosynthesis. Here we present physical evidence for a coenzyme Q-
biosynthetic polypeptide complex in isolated mitochondria. Separation of digitonin-
solubilized mitochondrial extracts in one-and two-dimensional Blue Native PAGE analyses
shows that Coq3 and Coq4 polypeptides co-migrate as high molecular mass complexes …
Saccharomyces cerevisiae at least eight mitochondrial polypeptides, designated Coq1–
Coq8, are required for Q biosynthesis. Here we present physical evidence for a coenzyme Q-
biosynthetic polypeptide complex in isolated mitochondria. Separation of digitonin-
solubilized mitochondrial extracts in one-and two-dimensional Blue Native PAGE analyses
shows that Coq3 and Coq4 polypeptides co-migrate as high molecular mass complexes …