Aminoglycoside suppression at UAG, UAA and UGA codons inEscherichia coli and human tissue culture cells
R Martin, AE Mogg, LA Heywood, L Nitschke… - Molecular and General …, 1989 - Springer
R Martin, AE Mogg, LA Heywood, L Nitschke, JF Burke
Molecular and General Genetics MGG, 1989•SpringerWe have compared the suppression of nonsense mutations by aminoglycoside antibiotics in
Escherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl
transferase (cat) gene, in the vector pRSV cat, were suppressed by growth in G418 and
paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme
assays for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and
during transient expression from the same plasmid in human 293 tissue culture cells. We …
Escherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl
transferase (cat) gene, in the vector pRSV cat, were suppressed by growth in G418 and
paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme
assays for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and
during transient expression from the same plasmid in human 293 tissue culture cells. We …
Summary
We have compared the suppression of nonsense mutations by aminoglycoside antibiotics inEscherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl transferase (cat) gene, in the vector pRSVcat, were suppressed by growth in G418 and paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme assays for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and during transient expression from the same plasmid in human 293 tissue culture cells. We have found significant differences in the degree of suppression amongst three UAG codons and two UAA codons in different mRNA contexts. However, the pattern of these effects are not the same in the two organisms. Our data suggest that context effects of nonsense suppression may operate under different rules inE. coli and human cells.
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