The plasma HDLs represent a major class of cholesterol-transporting lipoprotein that can be
divided into two distinct subfractions, HDL 2 and HDL 3, by ultracentrifugation. Existing
methods for the subfractionation of HDL requires lengthy ultracentrifugations, making them
unappealing for large-scale studies. We describe a method that subfractionates HDL from
plasma in only 6 h, representing a substantial decrease in total isolation time. The
subfractions so isolated were assessed for a variety of lipid and protein components, in …