Quantification of the major urinary metabolite of the E prostaglandins by mass spectrometry: Evaluation of the method's application to clinical studies
HW Seyberth, BJ Sweetman, JC Frolich, JA Oates - Prostaglandins, 1976 - Elsevier
Abstract Measurement of 7α-hydroxy-5, 11-diketotetranorprostane-1, 16-dioic acid,(PGE-M),
the major urinary metabolite of prostaglandin E 1 and E 2 in man provides a useful indicator
to monitor prostaglandin biosynthesis. For quantitative analysis of this prostaglandin
metabolite the stable-isotope dilution technique of selected ion monitoring (SIM) is
employed using gas-liquid chromatography-mass spectrometry. The preparation of the bis
(D 3-methyloxime), bis-methyl ester of PGE-M containing a tritium tracer in position 2 which …
the major urinary metabolite of prostaglandin E 1 and E 2 in man provides a useful indicator
to monitor prostaglandin biosynthesis. For quantitative analysis of this prostaglandin
metabolite the stable-isotope dilution technique of selected ion monitoring (SIM) is
employed using gas-liquid chromatography-mass spectrometry. The preparation of the bis
(D 3-methyloxime), bis-methyl ester of PGE-M containing a tritium tracer in position 2 which …