[HTML][HTML] Defensins from insects and plants interact with fungal glucosylceramides
K Thevissen, DC Warnecke, IEJA François… - Journal of Biological …, 2004 - ASBMB
Growth of the yeast species Candida albicans and Pichia pastoris is inhibited by RsAFP2, a
plant defensin isolated from radish seed (Raphanus sativus), at micromolar concentrations.
In contrast, gcs-deletion mutants of both yeast species are resistant toward RsAFP2. GCS
genes encode UDP-glucose: ceramide glucosyltransferases, which catalyze the final step in
the biosynthesis of the membrane lipid glucosylceramide. In an enzyme-linked
immunosorbent assay-based binding assay, RsAFP2 was found to interact with …
plant defensin isolated from radish seed (Raphanus sativus), at micromolar concentrations.
In contrast, gcs-deletion mutants of both yeast species are resistant toward RsAFP2. GCS
genes encode UDP-glucose: ceramide glucosyltransferases, which catalyze the final step in
the biosynthesis of the membrane lipid glucosylceramide. In an enzyme-linked
immunosorbent assay-based binding assay, RsAFP2 was found to interact with …