[HTML][HTML] The biosynthesis of mycolic acids in Mycobacterium tuberculosis: Enzymatic methyl (ene) transfer to acyl carrier protein bound meromycolic acid in vitro
Y Yuan, D Mead, BG Schroeder, YQ Zhu… - Journal of Biological …, 1998 - ASBMB
A closely related family of enzymes fromMycobacterium tuberculosis has been shown by
heterologous expression to catalyze the modification of mycolic acids through the addition of
a methyl (or methylene) group derived fromS-adenosyl-l-methionine (SAM). Overproduction
of all six of these enzymes in Escherichia coli and subsequent in vitro reactions with heat-
inactivated acceptor fractions derived from Mycobacterium smegmatis in the presence of
[methyl-3 H] SAM demonstrated that the immediate substrate to which methyl group addition …
heterologous expression to catalyze the modification of mycolic acids through the addition of
a methyl (or methylene) group derived fromS-adenosyl-l-methionine (SAM). Overproduction
of all six of these enzymes in Escherichia coli and subsequent in vitro reactions with heat-
inactivated acceptor fractions derived from Mycobacterium smegmatis in the presence of
[methyl-3 H] SAM demonstrated that the immediate substrate to which methyl group addition …