Insulin stimulates the translocation of the glucose transporter GLUT4 from intracellular
vesicles to the plasma membrane. In the present study we have conducted a comprehensive
proteomic analysis of affinity-purified GLUT4 vesicles from 3T3-L1 adipocytes to discover
potential regulators of GLUT4 trafficking. In addition to previously identified components of
GLUT4 storage vesicles including the insulin-regulated aminopeptidase insulin-regulated
aminopeptidase and the vesicle soluble N-ethylmaleimide factor attachment protein (v …