Publisher Summary This chapter focuses on the quantitation of receptor ligands by mass
spectrometry. It describes methods for the determination of oxysterols, LXR ligands, by gas
chromatography-mass spectrometry (GC-MS). Two of the methods include an optional
saponification step and purification on a disposable column prior to GC-MS analysis. GC-MS
is the method of choice for detection because of its specificity and sensitivity, which enables
the determination of compounds in complex mixtures over a large concentration range. A …