The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by …
J El Benna, LP Faust, BM Babior - Journal of Biological Chemistry, 1994 - ASBMB
The respiratory burst oxidase catalyzes the production of O2.-from oxygen and NADPH. It is
dormant in resting cells but becomes active when the cells are stimulated. Activation is
accompanied by the phosphorylation of multiple serines in the cytosolic oxidase component
p47phox, which moves from cytosol to the membrane during oxidase activation. Using
immunopurified p47phox isolated from 32Pi-loaded neutrophils activated with phorbol
myristate acetate, we showed that all the 32P was in the C-terminal CNBr fragment of the …
dormant in resting cells but becomes active when the cells are stimulated. Activation is
accompanied by the phosphorylation of multiple serines in the cytosolic oxidase component
p47phox, which moves from cytosol to the membrane during oxidase activation. Using
immunopurified p47phox isolated from 32Pi-loaded neutrophils activated with phorbol
myristate acetate, we showed that all the 32P was in the C-terminal CNBr fragment of the …