Relationship of protein phosphorylation to the activation of the respiratory burst in human neutrophils. Defects in the phosphorylation of a group of closely related 48 …
N Okamura, JT Curnutte, RL Roberts… - Journal of Biological …, 1988 - ASBMB
When 32P-labeled human neutrophils were activated by exposure to phorbol myristate
acetate, three 48-kDa proteins (designated pp48/6.8, pp48/7.3, and pp48/7.8, from their
isoelectric points) were found to have become labeled. With maximal stimulation, labeling
was complete by 30 s. With lesser degrees of stimulation, the extent of labeling at 2 min
correlated with rates of production by the phorbol-treated cells. Increased labeling of these
48-kDa proteins was also seen in cells exposed to f-Met-Leu-Phe. In phorbol-treated …
acetate, three 48-kDa proteins (designated pp48/6.8, pp48/7.3, and pp48/7.8, from their
isoelectric points) were found to have become labeled. With maximal stimulation, labeling
was complete by 30 s. With lesser degrees of stimulation, the extent of labeling at 2 min
correlated with rates of production by the phorbol-treated cells. Increased labeling of these
48-kDa proteins was also seen in cells exposed to f-Met-Leu-Phe. In phorbol-treated …