[HTML][HTML] Role of the small GTPase Rab7 in the late endocytic pathway
R Vitelli, M Santillo, D Lattero, M Chiariello… - Journal of Biological …, 1997 - ASBMB
Rab7 is a small GTPase localized to the late endosomal compartment. Its function was
investigated by overexpressing dominant negative or constitutively active mutants in BHK-21
cells. The effects of such overexpression on the internalization and/or degradation of
different endocytic markers and on the morphology of the late endosomal compartment were
analyzed. We observed a marked inhibition of the degradation of 125 I-low density
lipoproteins in cells transfected with the Rab7 dominant negative mutants while the rate of …
investigated by overexpressing dominant negative or constitutively active mutants in BHK-21
cells. The effects of such overexpression on the internalization and/or degradation of
different endocytic markers and on the morphology of the late endosomal compartment were
analyzed. We observed a marked inhibition of the degradation of 125 I-low density
lipoproteins in cells transfected with the Rab7 dominant negative mutants while the rate of …