[HTML][HTML] Identification of residues crucially involved in the binding of the heme moiety of soluble guanylate cyclase
PM Schmidt, M Schramm, H Schröder… - Journal of Biological …, 2004 - ASBMB
Soluble guanylate cyclase (sGC), a heterodimeric hemeprotein, is the only receptor for the
biological messenger nitric oxide (NO) identified to date and is intimately involved in various
signal transduction pathways. By using the recently discovered NO-and heme-independent
sGC activator BAY 58-2667 and a novel cGMP reporter cell, we could distinguish between
heme-containing and heme-free sGC in an intact cellular system. Using these novel tools,
we identified the invariant amino acids tyrosine 135 and arginine 139 of the β 1-subunit as …
biological messenger nitric oxide (NO) identified to date and is intimately involved in various
signal transduction pathways. By using the recently discovered NO-and heme-independent
sGC activator BAY 58-2667 and a novel cGMP reporter cell, we could distinguish between
heme-containing and heme-free sGC in an intact cellular system. Using these novel tools,
we identified the invariant amino acids tyrosine 135 and arginine 139 of the β 1-subunit as …