Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis
JP Buyon, SG Slade, EKL Chan, EM Tan… - Journal of …, 1990 - Elsevier
A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa
SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative
mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel
electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide
30: 08, ratio= 37.5. A higher ratio of monomer to cross-linker, ratio= 172.4 used in a 15%
acrylamide solution, readily separates the two peptide chains. This method facilitates the …
SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative
mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel
electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide
30: 08, ratio= 37.5. A higher ratio of monomer to cross-linker, ratio= 172.4 used in a 15%
acrylamide solution, readily separates the two peptide chains. This method facilitates the …