[HTML][HTML] Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase
Purine nucleoside phosphorylase catalyzes reversible phosphorolysis of purine nucleosides
and 2′-deoxypurine nucleosides to the free base and ribose (or 2′-deoxyribose) 1-
phosphate. Whereas the human enzyme is specific for 6-oxopurine ribonucleosides, the
Escherichia coli enzyme accepts additional substrates including 6-oxopurine
ribonucleosides, 6-aminopurine ribonucleosides, and to a lesser extent purine arabinosides.
These differences have been exploited in a potential suicide gene therapy treatment for …
and 2′-deoxypurine nucleosides to the free base and ribose (or 2′-deoxyribose) 1-
phosphate. Whereas the human enzyme is specific for 6-oxopurine ribonucleosides, the
Escherichia coli enzyme accepts additional substrates including 6-oxopurine
ribonucleosides, 6-aminopurine ribonucleosides, and to a lesser extent purine arabinosides.
These differences have been exploited in a potential suicide gene therapy treatment for …