Uridine 5′-diphosphate-glucose dolichyl-phosphate glucosyltransferase and bile acid
glucosyltransferase were quantitatively determined in subcellular fractions obtained by
differential centrifugation of human liver homogenate. Both enzymes were exclusively
enriched in the microsomal fraction with a recovery of total enzyme activity of 65.9±9.9% and
69.1±13.8%, respectively. Microsomal preparations were further subfractionated by
isopycnic centrifugation on a continuous sucrose density gradient. Both glucosyltransferases …