[HTML][HTML] Purification and characterization of the human interleukin-18 receptor
K Torigoe, S Ushio, T Okura, S Kobayashi… - Journal of Biological …, 1997 - ASBMB
Interleukin (IL)-18 was identified as a molecule that induces IFN-γ production and enhances
NK cell cytotoxicity. In this paper, we report upon the purification and characterization of
human IL-18 receptor (hIL-18R). We selected the Hodgkin's disease cell line, L428, as the
most strongly hIL-18R-expressing cell line based on the results of binding assays. This
binding was inhibited by IL-18 but not by IL-1β. The dissociation constant (K d) of 125 I-IL-18
binding to L428 cells was about 18.5 nm, with 18,000 binding sites/cell. After immunizing …
NK cell cytotoxicity. In this paper, we report upon the purification and characterization of
human IL-18 receptor (hIL-18R). We selected the Hodgkin's disease cell line, L428, as the
most strongly hIL-18R-expressing cell line based on the results of binding assays. This
binding was inhibited by IL-18 but not by IL-1β. The dissociation constant (K d) of 125 I-IL-18
binding to L428 cells was about 18.5 nm, with 18,000 binding sites/cell. After immunizing …