Interleukin-12 is expressed by infiltrating macrophages and synovial lining cells in rheumatoid arthritis and osteoarthritis
LI Sakkas, NA Johanson, CR Scanzello… - Cellular …, 1998 - Elsevier
TH1 cytokines have recently been detected in rheumatoid arthritis (RA) and osteoarthritis
(OA). For this reason we studied the TH-1-promoting cytokine IL-12 in synovial membranes
from patients with RA and OA. IL-12 transcripts and protein were analyzed by reverse
transcriptase–polymerase chain reaction (PCR) and immunohistochemistry, respectively. In
addition, IL-12 transcripts were quantitated by competitive PCR. IL-12 transcripts (p40) were
detected in 8 of 13 patients with RA and in 10 of 18 patients with OA. Their levels did not …
(OA). For this reason we studied the TH-1-promoting cytokine IL-12 in synovial membranes
from patients with RA and OA. IL-12 transcripts and protein were analyzed by reverse
transcriptase–polymerase chain reaction (PCR) and immunohistochemistry, respectively. In
addition, IL-12 transcripts were quantitated by competitive PCR. IL-12 transcripts (p40) were
detected in 8 of 13 patients with RA and in 10 of 18 patients with OA. Their levels did not …