Optimization of human T-cell expansion ex vivo using magnetic beads conjugated with anti-CD3 and Anti-CD28 antibodies
D Kalamasz, SA Long, R Taniguchi… - Journal of …, 2004 - journals.lww.com
T-cell receptor engagement and accompanying costimulatory signals control the level of
activation and functional potential of individual T cells. The authors previously developed a
novel technology in which human T cells are activated and expanded in culture ex vivo
using anti-CD3 and anti-CD28 monoclonal antibodies covalently linked to
superparamagnetic beads (Xcyte™ Dynabeads®). In this study the addition of N-acetyl l-
cysteine (NAC) to the cultures markedly increased the expansion of T cells from human …
activation and functional potential of individual T cells. The authors previously developed a
novel technology in which human T cells are activated and expanded in culture ex vivo
using anti-CD3 and anti-CD28 monoclonal antibodies covalently linked to
superparamagnetic beads (Xcyte™ Dynabeads®). In this study the addition of N-acetyl l-
cysteine (NAC) to the cultures markedly increased the expansion of T cells from human …