Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells

MA Morse, HK Lyerly, E Gilboa, E Thomas, SK Nair - Cancer research, 1998 - AACR
MA Morse, HK Lyerly, E Gilboa, E Thomas, SK Nair
Cancer research, 1998AACR
Dendritic cells (DCs), matured by CD40-ligand (CD40L), undergo marked changes in their
ability to process and present antigen, resulting in augmented lymphocyte stimulatory
activity. We demonstrate that the form of the tumor antigen (peptide or genetic material) used
to load the DCs dictates the required sequence of antigen loading and maturation for
antitumor immunotherapy. Optimal stimulation of carcinoembryonic antigen (CEA)-specific
CTLs by peptide-loaded DCs occurs when DCs from cancer patients are matured with …
Abstract
Dendritic cells (DCs), matured by CD40-ligand (CD40L), undergo marked changes in their ability to process and present antigen, resulting in augmented lymphocyte stimulatory activity. We demonstrate that the form of the tumor antigen (peptide or genetic material) used to load the DCs dictates the required sequence of antigen loading and maturation for antitumor immunotherapy. Optimal stimulation of carcinoembryonic antigen (CEA)-specific CTLs by peptide-loaded DCs occurs when DCs from cancer patients are matured with CD40L before exposure to CEA peptide, whereas optimal stimulation by RNA-transfected DCs occurs when the DCs are loaded with CEA RNA before maturation with CD40L.
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