High-fold expansion of human cytotoxic T-lymphocytes specific for autologous melanoma cells for use in immunotherapy
F Jotereau, MC Pandolfino, D Boudart… - Journal of …, 1991 - journals.lww.com
We set up a culture protocol that consistently allows high-fold expansion of tumor-specific T-
lymphocytes from most melanoma-invaded biopsies with low doses of recombinant
interleukin-2 (rIL-2). Between 2–60 x 10 6 T-lymphocytes could be obtained and
cryopreserved from 12 out of 13 patients, by culturing only 50 mm 3 tumor tissue with rIL-2.
Thawed lymphocytes from 11 of these patients could then be expanded by a median factor
of 32,800 by culturing them successively in microplates on irradiated feeder cells with rIL-2 …
lymphocytes from most melanoma-invaded biopsies with low doses of recombinant
interleukin-2 (rIL-2). Between 2–60 x 10 6 T-lymphocytes could be obtained and
cryopreserved from 12 out of 13 patients, by culturing only 50 mm 3 tumor tissue with rIL-2.
Thawed lymphocytes from 11 of these patients could then be expanded by a median factor
of 32,800 by culturing them successively in microplates on irradiated feeder cells with rIL-2 …