Determination of density distribution of red cell population

D Danon, Y Marikovsky - The Journal of laboratory and clinical …, 1964 - translationalres.com
Density distribution of cells (DDC) can be determined by the described method, in which
blood cells are separated according to their density using phthalate esters as separating
liquids. Methyl phthalate and di-n-butyl phthalate are mixed in different proportions to yield a
battery of fluids with increments of specific gravity of 0.004. The blood is introduced over the
different separating liquids in twenty microhematocrit capillaries. After centrifugation, denser
cells are separated from less dense cells by a transparent layer of the non-water miscible …