Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding
Y Matsuda, YN Harada, S Natsuume-Sakai… - … and Genome Research, 1992 - karger.com
Y Matsuda, YN Harada, S Natsuume-Sakai, K Lee, T Shiomi, VM Chapman
Cytogenetic and Genome Research, 1992•karger.comA technique for replication R-and G-banding of mouse lymphocyte chromosomes was
developed, and the replication R-banding pattern was analyzed. Optimal banding patterns
were obtained with thymidine-and BrdU-treatment of lymphocytes in the same cell cycle.
This produced replication R-band patterns that were the complete reverse of the G-band
patterns on all chromosomes. Replication R-banding methods can be used in conjunction
with nonisotopic, fluorescence in situ hybridization (FISH) to localize cloned probes to …
developed, and the replication R-banding pattern was analyzed. Optimal banding patterns
were obtained with thymidine-and BrdU-treatment of lymphocytes in the same cell cycle.
This produced replication R-band patterns that were the complete reverse of the G-band
patterns on all chromosomes. Replication R-banding methods can be used in conjunction
with nonisotopic, fluorescence in situ hybridization (FISH) to localize cloned probes to …
Abstract
A technique for replication R- and G-banding of mouse lymphocyte chromosomes was developed, and the replication R-banding pattern was analyzed. Optimal banding patterns were obtained with thymidine- and BrdU-treatment of lymphocytes in the same cell cycle. This produced replication R-band patterns that were the complete reverse of the G-band patterns on all chromosomes. Replication R-banding methods can be used in conjunction with nonisotopic, fluorescence in situ hybridization (FISH) to localize cloned probes to specific chromosomal bands on mouse chromosomes. With these methods the mouse complement factor H gene (cfh) was localized to the terminal portion of the F region of Chromosome 1. Q-banding patterns were also obtained by the replication R-banding method and may be useful for rapid identification of each chromosome.
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